Monticello is a city in Jefferson County, Florida, United States. The population was 2,506 at the 2010 census. It is the county seat of Jefferson County. The city is named after Monticello, the estate of the county's namesake, Thomas Jefferson, on which the Jefferson County Courthouse (Monticello, Florida) was modeled.
Monticello is home to Indian mounds and many historic buildings, including the Perkins Opera House and Monticello Old Jail Museum. The median income for a household in the city was $28,720.